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Allan Macfadyen was educated at Dr. Bryce’s collegiate school at Edinburgh from 1871. In 1878 he became a student in the University of Edinburgh. He graduated there M.B., C.M. (1883), M.D. with gold medal (1886), and B.Sc. in hygiene (1888). He studied chemistry and bacteriology in Berne, G√∂ttingen, and Munich.